The BSF operates several microscopes and image processing computers.
Before using the microscopes, you must sign up here to receive in-person training and to be added to the booking calendar (Exception: Light Microscope).
Zeiss Laser Scanning Microscope Confocal Microscope
- Captures multiple high-resolution 2-D images at various depths to reconstruct 3-D structures
- Provides a means of rejecting out-of-focus light from the detector to reduce blur
- Capable of fluorescence and bright-field microscopy
- Equipped with automated cell counter
- Equipped with filters for confocal imagery:
- DAPI (405 nm)
- GFP (488 nm)
- TxRed (594 nm)
- cy5 (635nm)
- Equipped with filters for fluorescence imagery:
- DAPI (405 nm)
- GFP (488 nm)
- Cy3 (555 nm)
- cy5 (635nm)
Zeiss Upright Fluorescent/Bright-Field Microscope
- Illuminates specimens with various pre-set wavelengths of light (excitation light) to visualize fluorescence emissions of fluorescent molecules or dyes in the sample
- Capable of slide scanning and image tiling
- Equipped with automated cell counter
- Equipped with a variety of filters:
- DAPI (405 nm)
- GFP (488 nm)
- Cy3 (555 nm)
- TxRed (594 nm)
- cy5 (635nm)
Upright Light Microscope
- Employs a light source to illuminate samples
- Equipped with a variety of lenses to provide imagery at several magnifications
- Among the most basic of microscopes
- Connected to a computer via HDMI for digital visualization and image-taking of samples
CSB Imaging Facility Microscopes
Located in the basement of the Ramsey Wright building, the Cell and Systems Biology (CSB) Imaging Facility operate a variety of microscopes:
- Scanning Electron Microscope (SEM)
- Transmission Electron Microscope (TEM)
- Spinning Disk Confocal Microscope (SDCM)
- Laser Scanning Confocal Microscope (Leica)
Please contact csb.reception@utoronto.ca to learn more about these microscopes.