Microscopes

Zeiss Laser Scanning Microscope Confocal Microscope

• Captures multiple high-resolution 2-D images at various depths to reconstruct 3-D structures
• Provides a means of rejecting out-of-focus light from the detector to reduce blur
• Capable of fluorescence and bright-field microscopy
• Equipped with automated cell counter
• Equipped with filters for confocal imagery:
  • DAPI (405 nm)
  • GFP (488 nm)
  • TxRed (594 nm)
  • cy5 (635nm)
• Equipped with filters for fluorescence imagery:
  • DAPI (405 nm)
  • GFP (488 nm)
  • Cy3 (555 nm)
  • cy5 (635nm)

Zeiss Upright Fluorescent/Bright-Field Microscope

• Illuminates specimens with various pre-set wavelengths of light (excitation light) to visualize fluorescence emissions of fluorescent molecules or dyes in the sample
• Capable of slide scanning and image tiling
• Equipped with automated cell counter
• Equipped with a variety of filters:
  • DAPI (405 nm)
  • GFP (488 nm)
  • Cy3 (555 nm)
  • TxRed (594 nm)
  • cy5 (635nm)

Upright Light Microscope

• Employs a light source to illuminate samples
• Equipped with a variety of lenses to provide imagery at several magnifications
• Among the most basic of microscopes
• Connected to a computer via HDMI for digital visualization and image-taking of samples
• Equipped with 5x, 10x, 20x, 40x, and 63x (oil) lenses